Dye-surface interactions were measured using fluorescence correlation spectroscopy (FCS). Methods such as plasma functionalization, PEGylation, or aminosilanization are commonly used to reduce nonspecific adsorption of biological molecules to glass or other surfaces.  Although most applications involve a desire to eliminate unwanted interactions, it is also important to consider the types of attractive forces present at the bare substrate and that are introduced by the presence of polymer brushes at interfaces.  Because of the sensitivity of single molecule analysis, it is important to be able to quantify the nature and extent of any interaction between the probe and the surface. The present work offers clarification of two classes of surfaces using FCS. The diffusion properties of two common fluorescent dye molecules (a cationic and an anionic dye) were used to quantify their interaction with the surface of a glass coverslip and a polymerized surface under various conditions.

Permeability of anti-fouling PEGylated surfaces probed by fluorescence correlation spectroscopy

239th National Meeting (2010)

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