Functional immobilization and lateralorganization of proteins into micro- and nanopatterns is an importantprerequisite for miniaturizing analytical and biotechnological devices. Here,we present a novel method for high contrast surface micropatterning using photodestructionof maleimide groups. This method is based on a bottom-up surface chemistry,which employed a poly(ethylene glycol) polymer brush as a biocompatible scaffold.The versatility of this method is demonstrated by means of different orthogonalfunctional groups for the purpose of site-specific protein immobilization usingcovalent- and affinity- based interactions. Furthermore, using maleimide basedalkyl-thiol chemistry, we utilized the patterning approach for capturing vesiclesand proteoliposomes onto surfaces. Formation of fluid patterned polymer-supportedmembranes was confirmed by lateral diffusion of lipids and proteins.

Maleimide-based functional protein micro-patterning

239th National Meeting (2010)

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