Backscattering interferometry (BSI) is a relatively new technique used to study molecular interactions in a label-free format and is proving to be a versatile biosensing technique.   BSI quantifies minute changes in refractive index created by a binding event to interrogate molecular interactions between proteins, ions and protein, and small molecules and proteins, with a high dynamic range of dissociation constants and unmatched sensitivity.   In this presentation, it will be shown that BSI can be used to study several new binding pairs, including membrane-associated proteins and antibodies for a variety of ligands.  We will discuss the investigation and quantification of the equilibrium binding constants in a label-free, solution-based environment and over a wide range of affinities.

Interrogation of label-free biomolecular interactions
utilizing backscattering interferometry

239th National Meeting (2010)

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Interrogation of label-free biomolecular interactions utilizing backscattering interferometry