As an important component of biological polysaccharides, glycosaminoglycans (GAGs) function as molecular co-receptors in numerous biological activities, such as cell�cell interaction, cell adhesion and migration, cell signaling, cell growth and differentiation. They mainly locate on the external side of cell membranes and contribute to the composition of extracellular matrix. Highly charged heterogeneous structure as they possess the sequence and composition of GAGs in different biological samples and the same biological samples under different conditions are always in the interests of researchers to understand glycobiology. Here we present our work in optimization of the composition of GAGs from biological samples�from improving extraction method to using different techniques for characterization, including reversed-phase ion-pair chromatography (R-IPC) coupled with electrospray ion mass spectroscopy (ESI-MS), strong anion exchange chromatography (SAX), capillary electrophoresis (CE) and introducing isotopically labeled disaccharide standards as internal standards for R-IPC coupled with ESI-MS.

Optimizatin of analysis of glycosaminoglycans in biological samples

240th National Meeting (2010)

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