SimCell� technology, a miniaturized bioreactor system, overcomesmany limitations encountered in traditional cell culture platforms. SimCellSystem enables running hundreds of micro-bioreactors in Design-of-Experimentlayouts with multiple process parameter control. Life Technologies uses SimCelltechnology to develop production media, feed supplements and fed-batchprocesses for recombinant mammalian cells. In the old paradigm, productionmedium is optimized in batch cultures and a suitable feed is then developed infed-batch mode. Using SimCell technology, we integrate media and feedoptimization workflow, which substantially reduces upstream process developmenttimeline. In the case study, production medium and feed for an Erythropoietin(EPO) producing Chinese Hamster Ovary (CHO) DG44 cell line is optimized within2 months. Sixteen media variants with different nutrient supplementation are formulatedin SimCell System and evaluated along with 3 diversified feeds. Statisticalanalysis is performed to identify the optimal basal medium and feed combinationthat promoted best growth and production.

SimCell™ technology:
Solution to high throughput (HTP) bioprocess development

240th National Meeting (2010)

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.