A Novel application of Ferrocene self-assembled monolayers (Fc-SAMs) for detection of molecular interactions is described. Our Fc-SAM contains three different head groups: -OH, -Fc and the receptors (biotin).  Upon binding, the intrinsic electric charge of the target (streptavidin) modifies the electrostatic potential at the plane of electron transfer, and causes a shift in the formal potential Eo'. The measured Eo' shift vs. pH shows good agreement with the streptavidin theoretical titration curve (figure 1). Other probe-target interactions will be presented and analysed theoretically on the basis of a published model1. A new method to extract the standard electrode potential of the electro-active SAM from Eo' vs. ionic strength, will be also presented.  (1) Smith, C. P.; White, H. S. Anal. Chem. 1992, 64, (20), 2398-2405.

Detection of molecular interactions with modified ferrocene self-assembled monolayers

240th National Meeting (2010)

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