The present study aimed to elucidate post mortem changes in fish muscle tissues by tracing changes in the gene expression of rainbow trout, Oncorhynchus mykiss, and Japanese jack mackerel, Trachurus japonicus. The fish were stored in ice for up to 5 days after death and the expression of the mRNA and specific cDNA in the ordinary muscle tissues was analyzed by a Real-time reverse transcriptase polymerase chain reaction analysis. Initial changes in the concentrations of freshness related biochemical constituents were also determined. The results showed that troponin I and glyceraldehyde-3-phosphate dehydrogenase mRNAs increased after 3h and 6h, respectively, and then they gradually decreased. On the other hand, ATP concentrations decreased simultaneously with the rise of gene expression. These results suggest that the increased mRNAs in fish muscle occurred by transcription immediately after death.

Post mortem changes in gene expression of fish muscle tissues

240th National Meeting (2010)

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