Application of trans-retinoic acid (RA) induces neuronal differentiation of pluripotent cells. The synthesis of RA by retinaldehyde dehydrogenase is important in pre-patterning in the early embryo. Anterior-posterior mapping is directed by RA via Hox signaling. The changes in protein and mRNA expression and in the glycolyx can be tracked as the cells differentiate down the neuroprogenitor pathway under the influence of RA using LC-MS, QT-PCR, immunohistochemistry, a method of in vivo Western blotting that has been developed in our lab, and conventional Western blotting. These data, obtained using NCC-IT, a pluripotent embryonal carcinoma, model the activity of the blastocyst and data from human embryonic stem cells.

Analysis of<i> trans-</i>retinoic acid - induced neuronal differentiation of pluripotent embryonal carcinoma cells

240th National Meeting (2010)

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Analysis of trans-retinoic acid - induced neuronal differentiation of pluripotent embryonal carcinoma cells