We have developed acapillary chromatography system using an open capillary tube made offused-silica, polyethylene, or poly(tetrafluoroethylene), and a water-hydrophilic-hydrophobicorganic mixture carrier solution. This tube radial distribution chromatography(TRDC) system works under laminar flow conditions. In this study, the followinganalytical conditions in the TRDC system using a fused-silica capillary tube anda water-acetonitrile-ethyl acetate mixture carrier solution were examined: tubetemperature, tube inner diameter, tube effective length, and flow rate. Theresolution, theoretical plate number, and height equivalent to the theoreticalplate were calculated from the experimental data obtained by examining theeffects of tube length. The mixture of 1-naphthol, Eosin Y,1-naphthalenesulfonic acid, 2,6-naphthalenedisulfonic acid, and1,3,6-naphthalenetrisulfonic acid was subjected to the present TRDC system andthe analytes in the mixture solution were eluted in this order with the organicsolvent-rich carrier solution, providing good separation performance on thechromatogram.

Capillary chromatography based on tube radial distribution of water-acetonitrile-ethyl acetate mixture carrier solvents under laminar flow conditions

240th National Meeting (2010)

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