An emerging concept in cell signallingis the natural role of reactive oxygen species such as hydrogen peroxide (H2O2) as beneficialmessengers in redox signalling pathways. In spite of the growing evidence, thenature of H2O2 signalling isconfounded by difficulties in tracking it in living systems, both spatially andtemporally, at low concentrations. Here, we develop an array of fluorescentsingle-walled carbon nanotubes that can selectively record, in real time, thediscrete, stochastic quenching events that occur as H2O2 molecules are emittedfrom individual human epidermal carcinoma cells stimulated by epidermal growthfactor.(Jin, Heller et al. 2008; Jin, Heller et al. 2010) We show mathematically that sucharrays can distinguish between molecules originating locally on the cellmembrane from other contributions. We find that epidermal growth factor induces2 nmol H2O2 locally over a periodof 50 min. This platform promises a new approach to understanding thesignalling of reactive oxygen species at the cellular level. We have alsoextended this approach to the development of a single molecule detection platformfor nitric oxide.

Detection of single-molecule H<sub>2</sub>O<sub>2</sub>
signalling from epidermal growth factor receptor using fluorescent
single-walled carbon nanotubes

240th National Meeting (2010)

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Detection of single-molecule H2O2 signalling from epidermal growth factor receptor using fluorescent single-walled carbon nanotubes

Detection of single-molecule H₂O₂ signalling from epidermal growth factor receptor using fluorescent single-walled carbon nanotubes