Heparosan is an acidic polysaccharide natural product.  It is a critical precursor in heparin biosynthesis and in the chemoenzymatic synthesis of bioengineered heparin. The process of heparosan production by E. coli K5 fermentation significantly affects the cost and the pharmacological properties of the final product bioengineered heparin. In this study, heparosan produced by E. coli K5 fermentation was characterized and the fermentation process was optimized and controlled to produce the ideal bioengineered heparin precursor. E. coli strain improvement by metabolic engineering and gene manipulation was also investigated.

<i>E. coli</i> K5 heparosan production for bioengineered heparin

240th National Meeting (2010)

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E. coli K5 heparosan production for bioengineered heparin