As the threat of an influenza pandemic has become more apparent, there is an increasing need for responding quickly to mass produce influenza vaccine. The shift from egg-based to cell culture-based vaccine production is seen as one way to secure a more rapid response. Furthermore, one additional obstacle is to overcome the need to greatly reduce time for clinical trials & approval i.e. to reduce time to market, where cleaning validation is of major importance. Here, we present a way for eliminating cleaning validation by using a disposable bioreactor alternative. We have performed an optimization study on production of live Influenza A/Solomon Islands/3/2006 in Vero cells by using Cytodex� microcarriers in WAVE Bioreactor� systems. Parameters such as inoculation conditions, additives and composition of media etc. have been considered. Thus, the data shows that production of the influenza virus in WAVE Bioreactor� system is a fast and convenient alternative to conventional systems.

Optimization study on propagation of Influenza A virus in Vero cells by using Cytodex™ microcarriers in WAVE Bioreactor™ systems

241st National Meeting (2011)

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