Heparin is potent anticoagulant comprised of long, unbranched, anionic polysaccharide chains.  Although its basic structure consists of repeating disaccharides of uronic acid and glucosamine disaccharides, heparin is microhetergeneous due to various patterns of sulfonation and the presence of uronic acid epimers.  The chemical properties of different oligosaccharide chains containing glucuronic and iduronic acid, which differ only by C5 epimerization, will be investigated.  Although iduronic acid is found in greater abundance in heparin, glucuronic acid is an important constituent and is required for antithrombin III binding.  Differences in the chemical properties of oligosaccharide chains containing iduronic and glucuronic acid residues will be examined through their chromatographic retention behavior and carboxylate pKa values determined by 1H NMR pH titration.  The pH of oligosaccharide solutions will be indirectly determined through the chemical shifts of formate and acetate NMR indicators.

Analysis of iduronic and glucuronic acid residues in heparin polysaccharide chains

241st National Meeting (2011)

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