In this presentation, we report the facile preparation of glycogen-based hydrogel by phosphorylase-catalyzed chain-elongation of glycogen using glucose 1-phosphate. Moreover, lyophilization of the resulting hydrogel afforded the glycogen-based xerogel. The X-ray diffraction profile of the xerogel showed that the network structure in the xerogel was constructed based on the double helical entanglement of the elongated amylose chains, which in turn supported presence of the junction zones by the double helix formation in the hydrogel. The re-hydrogelation and film formation from the xerogels were easily accomplished by the appropriate techniques. Highly branched anionic polysaccharides were also synthesized by the thermostable phosphorylase-catalyzed transfer reaction of glucuronic acid residues to cluster dextrin or glycogen using glucuronic 1-phosphate as the substrate.

Synthesis of new polysaccharide materials by phosphorylase-catalyzed chain-elongation

241st National Meeting (2011)

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