Understanding microbial degradation of estrogens is critical in predicting the fate of estrogens in the environment and treating estrogen contaminated water/wastewater. A bacterial strain capable of degrading E2 was isolated from activated sludge and identified as Stenotrophomonas maltophilia. A quantitative proteomic study of E2 degradation by S. maltophilia was carried out using 2-D LC-MS/MS, and followed by database analysis using S. maltophilia R551 genome in MASCOT. Results showed that after a 4-hr exposure to E2, key anabolism and catabolism pathways were highly impacted. Moreover, homologs of five enzymes responsible for testosterone degradation by Comamonas testosteroni TA441 was also identified with three of them up-regulated by >2 folds after the exposure to E2. Our results show effects of E2 exposure on S. maltophilia metabolism and suggest an overlap between the degradation pathways for testosterone and estradiol. These results are instructive in enhancing estrogen biodegradation in water/wastewater treatment.

Proteomic analysis of 17β-estradiol (E2) degradation by <i>Stenotrophomonas maltophilia</i>

241st National Meeting (2011)

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Proteomic analysis of 17β-estradiol (E2) degradation by Stenotrophomonas maltophilia