Simple microfluidic-based techniques to quantitate the binding affinities between the glycopeptide antibiotics vancomycin and teicoplanin to fluorescently labeled D-Ala-D-Ala terminus peptides are described. In this work, 3-aminopropyltriethoxysilane (APTES) is used to modify the surfaces of a series of microchannels and each channel is subsequently exposed to a solution of antibiotic for a few minutes. The antibiotic is retained after washing through electrostatic interactions and the series of channels are subsequently exposed to an increasing concentration of peptide followed by washing to exclude any non-specific binding. Extent of fluorescence is quantified using a microscope fitted with a CCD camera. Binding constants were determined to be in good agreement with previous data. Modifications in the technique are explored. The ease of quantifying extent of interaction in this microchip technique may prove powerful for exploration of a myriad of receptor-ligand pairs.

Development of microfluidic chip-based assays for heterogeneous receptor-ligand interaction studies

241st National Meeting (2011)

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