Herein we report on the encapsulation of proteins into triggered release nanoparticles.  Nanoparticles were prepared from a novel dual-response polymer using high pressure homogenization1. Nanoparticles were prepared using double emulsion method incorporating a model protein. Tangential flow was used to purify the resulting nanoparticles.  The particles were characterized by dynamic light scattering (DLS) in pH 7.4 and 5. Protein integrity was analyzed by gel electrophoresis and measuring its ultravilot and fluorescence emission spectrums and was found to be stable. Degradation kinetics show that these nanoparticles have the ability to release their encapsulated payload when exposed to mildly acidic pH resembling the pH levels of early endosomes and diseased tissue. Furthermore, the nanoparticles were stable for 24 hours in normal cellular pH of 7.4.  Our results indicate the potential of this vehicle to deliver therapeutics and diagnostics to cells and diseased tissue.1Sankaranarayanan et al, ACS Nano, Article ASAP DOI: 10.1021/nn100968e.

Encapsulation of protein into a biodegradable polymer using high pressure homogenization

241st National Meeting (2011)

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