Recovery of protein from inclusion bodies is studious, timeconsuming and development of such protocols sometimes impossible. Thereforethere is strong need for the development of strategies to mitigate theformation of inclusion bodies during over expression of proteins in bacterialhosts. In this study we expressed the FGFR and tested the effect of gold on theformation of inclusion in the bacterial host system. We treated the E. coli cellswith increasing concentrations of gold and characterized the growth usingUV-Visible spectrum. Expression and solubility of the protein was further analyzedusing SDS-PAGE electrophoresis. Proteins recovered from the soluble fractionwas, further purified using the Nickel affinity column chromatography. Thefindings of the present study pave way for recovery of other biologicalmacromolecules from insoluble inclusion bodies.

Nanoparticle assisted recovery of recombinant proteins from insoluble form

241st National Meeting (2011)

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