A double-blind, placebo-controlledpilot clinical trial on the effect of cranberry juice cocktail (CJC) consumptionon biofilm formation and bacterial adhesion was conducted in 10 healthy womenbetween the ages of 18 and 27. A single dose of 16 oz. of CJC or a placebo beveragewas given to the volunteers, and urine samples were collected in the following48 hours. Bacteria (E. coli B37,CFT073, BF1023, HB101, and S. aureus ATCC43866)were cultured in the urine samples supplemented with media and the amount ofbiofilm formed was measured using a crystal violet absorbance assay in a96-well plate. In the urine of volunteers who had consumed CJC, biofilmformation was inhibited within 24 hours after CJC consumption, and biofilmformation started to increase after 48 hours by 49-67%. S. aureus showed the least biofilm formation after incubation withpost-CJC urine. While E. coli CFT073is a commonly studied uropathogenic E.coli strain and it expresses P fimbriae, E. coli B37 and BF1023 are antibiotic resistant and do not containP fimbriae.  Atomic force microscopymeasurements of bacterial adhesion also revealed that cranberry metabolitescould block bacterial adhesion, and changed the conformation of molecules onthe bacterial surfaces. This is the first study to show that biofilm formationin antibiotic resistant E. coli and awide range of uropathogens, including S.aureus, can be prevented in the urine of healthy women who have consumedCJC. We also demonstrated the duration of the benefit, noting that anti-biofilmactivity in the urine peaks between 24 and 48 hours after drinking CJC,depending on the bacterial strain studied.

Prevention of biofilm formation and bacterial adhesion after consumption of cranberry juice cocktail

242nd National Meeting (2011)

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