We introduce a microcapillary microfluidic technology for the fabrication of compositionally asymmetric giant unilamellar vesicles (GUVs). Monodispersed water in oil in water (w/o/w) double emulsion droplets were formed in a single step in a glass microcapillary system. Lipid vesicles were formed after removal of the oil phase by evaporation. By incorporating different small unilamellar vesicles (SUVs) into each of the water phases, an asymmetric membrane can be fabricated, with compositionally distinct lipid monolayers formed at each water-oil interface. Lipid bilayer membrane asymmetry is confirmed by binding of labeled annexin V to membranes formed with an asymmetric composition of phosphatidylserine.

Microcapillary microfluidic fabrication of asymmetric giant lipid vesicles with minimal organic solvent

243rd National Meeting (2012)

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