Silver nanoparticles have been used in several biological, technological and medical applications. It is already known that the size of nanoparticles play an important role in their electrical, optical and magnetic properties. Simple syntheses methods allow us to prepare nanoparticles controlling their size and inhibit growth are object of study. Our research focused in synthesis of silver nanoparticles for their application as biosensors. We described the preparation of silver nanoparticles using different reducing agents. ascorbic acid, glucose, sodium borohydride and sodium citrate were the reducing agents selected for the study. A variety of colors like yellow, amber, gray and translucent were achieved. Ultraviolet-visible spectroscopy was performed for to confirm the formation of silver nanoparticles. The analyzed samples show an absorption band center between 380nm and 430 nm. Yellow nanoparticles were selected to perform the interaction with functional groups and biomolecules. After the addition of selected amounts of biomolecules to colloidal nanoparticle samples, important changes in the absorption band were observed. The study was performed with urea, cysteine, glycogen, dextrose and lecithin.Keywords: Nano/Materials, nanoparticles, aminoacids, optical properties

Synthesis and characterization of silver nanoparticles for biosensors design

243rd National Meeting (2012)

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