There has been a growing interest in surface tailoring for the purposes of immunoassays and DNA detection of biosensors, artificial or intelligent membranes, molecular electronic devices and novel materials. This paper describes an approach to developing repeatedly regenerable time-resolved fluorescent chelating surface, which is based on complexation and dissociation interactions. As a Europium (III, Eu3+) chelator, BCPDA (abbreviated from 4, 7-bis (chlorosulfophenyl)-1, 10-phenanthroline-2, 9-dicarboxylic acid) and its amine derivatives were synthesized in facile procedures. The BCPDA and its derivatives could covalently be attached onto the amino-surfaces of silanized glasses, silica and polymer membrane under relatively mild conditions, generating BCPDA-derivatized coatings capable of chelating Eu3+. Subsequent incubation with Eu3+ solution initiated complexation to give time-resolved fluorescent surfaces, thus the fluorescence spectra and related lifetimes were determined. Further immersion of complexed surfaces in acidic solution regenerated chelating coatings as a result of Eu3+ dissociation. These results implied promising applications in display and anti-falsification materials, surface micropatterned devices and biomolecules labeling and detection based on such principle of complexing interactions.

Europium (III) chelating coatings with tunable time-resolved fluorescence properties on amino-surfaces

243rd National Meeting (2012)

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