The DNA origami method developed for the preparation of fully addressable two-dimensional (2-D) structures has been utilized for the selective positioning of the functional molecules and nanoparticles and for the design of various 3-D architectures. We recently developed �DNA frame� using the DNA origami method to examine enzymatic action. In our DNA frame, tensed and relaxed dsDNA can be created by bridging the defined length of dsDNA in the DNA scaffold. The relaxed strand can accommodate the enzymes to bind and bend the target sequences. On the other hand, the tensed strand allows binding of these enzymes, while this strand is a poor substrate for bending, resulting in the lower reaction efficiency. In addition, the DNA frame is valuable for analyzing the motion of the enzyme because of the defined coordinated space. In this presentation direct observation of DNA modifying enzymes will be presented.

Direct observation of single molecular event in DNA origami frame

243rd National Meeting (2012)

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