The use of bacterial surface proteins like Protein A and L can be highly effective for the purification and detection of antibodies. However, there are gaps in the functionality that they provide, and when a target protein falls into one of these gaps, the process of finding an alternative strategy can be extremely frustrating. For this reason BAC has developed a range of �CaptureSelect� affinity ligands, derived from heavy chain antibodies found in camelids, directed against a unique panel of antibody sub-domains, like IgG-CH1, - CH2, - CH3, kappa - and lambda light chains. When conjugated to biotin, excellent compatibility with different streptavidin based biosensors is demonstrated providing  researchers valuable tools for label free detection, quantitation and characterization of in principle any antibody format (e.g. Fab fragments). Novel analytical concepts will be described that accelerate downstream process development and enable monitoring of product quality during manufacturing.

Introducing single domain antibody based affinity ligands for antibody detection and characterization in label free binding platforms

243rd National Meeting (2012)

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