In recent years Amgen, has incorporated chemically defined production media and feeds into their mammalian platform for recombinant protein expression.  In this study, the impact on expression and product quality was examined at the 2kL scale for a monoclonal antibody. Results are shown for three chemically defined production and feed media, each from a different stage of a performance improvement development cycle.  The study found that reformulated production media and feeds improved specific productivity, titer, and processing of the signal peptide for the heavy chain of the antibody product.  The final reformulated media has essentially the same ingredients as the original formulation, but the amount of different ingredients in the culture at a given time has significant impact on product quantity and quality.

Chemically defined media development improves signal peptide processing:  A case study at the 2kL scale

243rd National Meeting (2012)

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