Mediated enzyme reactions form the basis of many applications, ranging from biosensors, through biocatalytic fuel cells, to applications in industrial electrolysis. Redox mediators can be used to shuttle electrons between enzyme and electrode. The mediation can be tailored to redox enzymes with appropriate matching of redox potentials.Here we present our research on tailoring surfaces through a combination of surface treatment and engineering and anchoring of polymeric supports, to provide a matrix for co-immobilisation of redox mediator and enzyme. We have combined a library of osmium-based functionalised redox mediator complexes with redox enzymes that can catalyse reactions of interest in biofuel cells.Cyclic voltammetry of solutions of mediator and enzyme is used to screen for the most suitable combination providing high currents at appropriate potentials for subsequent immobilisation.We present our most recent results using different approaches aimed at improving the stability and power output of biofuel cells and biosensors.

Mediated enzyme electrochemistry: Signal output and stability enhancement using tailored mediators

243rd National Meeting (2012)

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