Membrane adsorption chromatography has become a popular downstream purification step for monoclonal antibodies.  This technology offers advantages such as disposability, high capacity, and ease of use.  Mechanistically, adsorbers are believed to function similar to flow-through mode anion exchange chromatogarphy.  In this scenariom the electrostatic environment is such that the desired product of interest passes through the media, while undesired contaminants do not.  We performed a series of experiments to assess the comparability of adsorbers with anion exchange.  Using a model antibody feedstock, we assed the ability of both operations at small-scale to retain host cell DNA and protein, and viruses.

Comparison of adsorbtive membranes to traditional anion exchange chromatography as an alternative purification step for monoclonal antibodies

243rd National Meeting (2012)

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