A whispering gallery mode (WGM) biosensor with an integrated flow cell was used to measure the adsorption kinetics of fibronectin (FN) on three different alkysilane self-assembled monolayers (SAMs) down to the lowest solution concentrations yet reported.  On hydrophilic polyethylene glycol silane (SiPEG) surfaces and at high solution concentrations on hydrophilic aminated (DETA) and hydrophobic perfluorinated (13F) silanes, experimental results generally agreed with published results.  At solution concentrations of 1 �g/ml and below, significantly higher adsorption densities were measured on DETA and 13F compared to alkanethiol SAMs with similar properties.  The surface density of adsorbed FN was similar for DETA and 13F surfaces.  Mathematical models fitted to the experimental results indicated that FN denatured after adsorption on 13F, but not on DETA.   The surface structure and subsequent biological function of adsorbed FN were confirmed by culturing embryonic hippocampal neurons and skeletal myoblasts on FN-coated silane surfaces.

Adsorption of fibronectin on alkylsilane monolayers and its effect on cell adhesion and growth as compared to thiols on gold

243rd National Meeting (2012)

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