PEBP (PhosphatydilEthanolamine Binding Protein), more recently renamed RKIP (Raf Kinase Inhibitor Protein), is the product of a metastasis suppressor gene. It is known that one covalent ligand, locostatin, increases PEBP/RKIP's ability to inhibit cell migration. PEBP/RKIP also possesses a nucleotide binding site, in particular it can non-covalently bind FMN, GTP, UMP and CTP.  Aiming to characterize the non-covalent RKIP/PEBP-nucleotide interaction, we have designed and synthetize new FMN analogues and analyze the complex PEBP/RKIP in native conditions by ESI-IT mass spectrometry. Four groups of nucleotide analog ligands were distinguished according to their ability to bind human PEBP/RKIP.

Synthesis of FlavinMonoNucleotide analogs targeting noncovalent interactions with PEBP/RKIP protein

243rd National Meeting (2012)

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