High-performance liquid chromatographic method has been developed in reversed-phase conditions for the separation of racemic carvedilol, (�)-1-(9H-carbazol-4-yloxy)-3-[[2-[2-methoxyphenoxy)ethyl]amino]-2-propanol, an anti-hypertensive agent with non-selective �- and a1-adrenegic receptor blocking activities in bulk drug. In this method mobile phase system consists of 25 mM ammonium acetate pH 6.5 with formic acid: methanol (10:90 v/v) and amylose based Chiralpak AD-RH (150 x 4.6 mm, 5 �m) column. The conditions affording the best resolution (3.14) were found by selection and variation of the mobile-phase composition and the difference in separation capability of the method is noted. Relative standard deviation of retention time and peak areas were found to be 0.2% & 0.3 %, respectively for precision. Carvedilol sample solution and mobile phase were found to be stable for 48 hr.

High performance liquid chromatographic method for the separation of racemic Carvedilol

243rd National Meeting (2012)

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