To increase column resilience in high pH and temperature environments, we have developed diamond core-shell particles for High Pressure Liquid Chromatography (HPLC). Standard HPLC columns are limited to a pH range of 2 to 8 and operate at temperatures below 40 �C. However, high temperatures are beneficial because they allow for faster elution times with minimal loss in efficiency. Furthermore, many pharmaceuticals are basic in nature, and therefore would benefit from a basic environment for analysis. Such basic environments would destroy standard silica based HPLC particles. Most recently, we have investigated epoxide and amine chemistry on a diamond core-shell to overcome the limitations presented by the current materials for HPLC. There are, however, some drawbacks to such chemistry. For example, the amine/epoxide chemistry creates hydroxyl groups, which can participate in secondary reactions between analytes and the stationary phase. These secondary interactions may lead to tailing in the chromatogram. Herein, we examine a subset of the possible cross-linking chemistries that will provide us with the robust particles we desire.

New functionalizations of diamond-based pellicular particles for HPLC

243rd National Meeting (2012)

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