The objective of this study was to develop new strategies for the controlled attachment of antibodies on cellulose and to optimize the surface properties for minimized non-specific binding of target molecules. The surface modification of cellulose was performed by using polysaccharide adsorption and/or chemical functionalization. Consequent attachment of antibodies was achieved either by directly conjugating them onto the functionalized cellulose or by using a specific linker protein that allowed for chemical free attachment strategy. For example, antibodies such as monoclonal anti-hemoglobin and polyclonal anti-human IgG were irreversibly attached to cellulose. Developed platforms were found to be effective on detecting specific antigens with reduced non-specific binding. XPS, QCM-D, SPR and AFM were used to characterize the main chemical, swelling and morphological features of the produced, novel biointerfaces based on the associated, derivatized cellulosic materials. Main results and possible applications for such systems will be discussed.

Development of cellulose based biointerface for diagnostic and affinity filtration applications

243rd National Meeting (2012)

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