Glycosyltransferases are key enzymes for creating diverse arrays of carbohydrate structures that mediate many biological processes. Many glycosyltransferases that are responsible for the synthesis of carbohydrate-containing structures requires uridinediphospho-sugar donors. In attempts to explore the promiscuity of various glycosyltransferases toward their UDP-sugar donors, a highly efficient one-pot multienzyme system containing a uridylyltransferase, an inorganic pyrophosphatase, and/or a kinase is developed and applied to quickly attain structurally defined UDP-glucose, UDP-galactose, UDP-mannose, and their derivatives from a free monosaccharide or  commercially available inexpensive monosacchhardie-1-phosphate. These sugar nucleotides have been used as donor substrates of several glycosyltransferases for the synthesis of complex carbohydrates.

Highly efficient one-pot multienzyme system for the synthesis of UDP-GLC(GAL)(MAN) and derivatives

243rd National Meeting (2012)

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