At the College of Saint Benedict/St. John's University, we have received NSF funding to fully revise our Foundation laboratory sequence.   We have developed a sequence of integrated labs that develop purification, chromatography and synthesis techniques from the fields of organic, inorganic and biochemistry.    The third laboratory course in the sequence, �Synthesis�, requires the students to learn synthesis techniques including the induced synthesis of proteins in E. Coli (i.e. expression).  In this new experiment, students use one of the commercially available plasmids for green (GFP), blue (BFP), banana (pmBFP), or cherry (pmCFP) fluorescent proteins to transform competent JM109 E. Coli cells.   After transformation and expression, students analyze their FP solution by a hand-held UV light to detect fluorescence and by SDS-polyacrylamide gel electrophoresis.  Details of the experiment and its context within the development of a new Foundation Synthesis Laboratory will be addressed.

Synthesis and characterization of variant fluorescent proteins in <i>E. Coli</i> in a second year foundation synthesis

243rd National Meeting (2012)

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Synthesis and characterization of variant fluorescent proteins in E. Coli in a second year foundation synthesis