We here report fluorescence-based binding assay of hydrophobic DNA to the lipid bilayer membrane. Hydrophobic DNA consists of a hydrophobic region corresponding to the thickness of the lipid bilayer membrane and hydrophilic regions at both ends. The synthesis of the hydrophobic DNA was achieved by ultra-mild phosphoramidite method. In order to investigate interaction between the hydrophobic DNA and 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) liposome, we prepared hydrophobic DNA containing a dielectric-sensitive probe. Fluorescence of the hydrophobic DNA was enhanced and blue-shifted by the presence of POPC liposome. These results indicate that the hydrophobic DNA resided in the hydrophobic region of the liposome.

Fluorescence-based binding assay of hydrophobic DNA to the lipid bilayer membrane

243rd National Meeting (2012)

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