Perfluoroalkyl Acids (PFAAs) are used in the manufacture of a variety of consumer products, and their presence in the environment is a growing environmental and toxicological concern. They have been shown to bioaccumulate in the liver and blood stream of many organisms. In this study, the binding of PFAAs to human serum albumin (HSA) over a range of differing pH and ionic strength values was investigated using two experimental approaches. Fluorescence spectroscopy and 19F nuclear magnetic resonance (19F NMR) spectroscopy were used to qualitatively and quantitatively describe the binding of PFAAs with varying chain length and ionic head group to HSA as a function of pH and ionic strength. Fluorescence results indicate that ionic strength has little effect on the binding, while pH has a greater effect. Estimated binding constants from these studies are on the order of 104 M-1, varying with fluorocarbon chain length, ionic head group, and pH.

Investigation of the binding of perfluoroalkyl acids to human serum albumin through <sup>19</sup>F nuclear magnetic resonance and fluorescence spectroscopy

243rd National Meeting (2012)

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Investigation of the binding of perfluoroalkyl acids to human serum albumin through 19F nuclear magnetic resonance and fluorescence spectroscopy

Investigation of the binding of perfluoroalkyl acids to human serum albumin through ¹⁹F nuclear magnetic resonance and fluorescence spectroscopy