Poly (ethylene oxide)-like glow discharge plasma polymerized tetraglyme surfaces prepared at low power (10W) display substantial in vivo monocyte/macrophage adhesion and a typical foreign body reaction, despite having very low fibrinogen adsorption (GFg). GFg measured under more rigorous conditions was substantially above the ultralow level needed to prevent cell attachment (63 or 55 ng/cm2 from 3 mg/ml Fg in buffer or 100% blood plasma respectively), but the increased GFg did not correlate well to monocyte adhesion. The availability of the Fg epitope at ?377-395 responsible for monocyte adhesion, measured using monoclonal antibody P2 binding, showed a strong linear correlation with adhesion. However, P2 binding per unit of adsorbed Fg was much greater for Fg on 10W tetraglyme than on control surfaces with higher GFg (80W tetraglyme or FEP) suggesting Fg adsorbed to this nonfouling surface may be a less denatured state that increases its potency towards monocytes.

Fibrinogen adsorption to plasma discharge deposited tetraglyme is ultralow but exhibits higher binding of monoclonal antibodies to the macrophage binding epitope than higher adsorbing
surfaces

243rd National Meeting (2012)

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Fibrinogen adsorption to plasma discharge deposited tetraglyme is ultralow but exhibits higher binding of monoclonal antibodies to the macrophage binding epitope than higher adsorbing surfaces