Cellulosomes are multi-enzyme complexes responsible for efficient degradation of plant cell wall polysaccharides. The non-enzymatic scaffoldin subunit provides a platform for cellulolytic enzyme binding that enhances the overall activity of the bound enzymes. Understanding the unique quaternary structural elements responsible for the enzymatic synergy of the cellulosome is hindered by the large size and inherent flexibility of these multi-protein complexes. Our recent work has combined a suite of complementary structural biology and biophysical approaches (i.e. NMR spectroscopy, X-ray crystallography, small angle X-ray scattering, and molecular dynamics) to characterize the modular organization, dynamics, and higher order structure of the scaffoldin subunit and enzyme-containing multi-modular cellulosomal fragments.

Cellulosome dynamics and higher order structure

243rd National Meeting (2012)

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