Nitric oxide (NO) is an important cellular signaling molecule involved in many biological processes in many organisms and also in human beings. Furoxans have been tested to release NO in the presence of thiol cofactors. Different disubstitutedbenzoylfuroxans were examined to inspect the amount of NO that can be released at physiological pH. The Furoxans were dissolved in dimethyl sulfoxide(DMSO) and reacted with L-cysteine to form an s-nitroso intermediate. After incubatation the intermediate mixure reacted with mercury(II) chloride(HgCl2) to release NO which subsequently formed fluorescent product with a 2,3-Diaminonaphthalene(DAN) reagent.  The relationship of fluorescence intensity and the amount of furoxan used was generated with Nanodrop 3300 fluorospectrometer.  The quantitative NO releases from various dibezoyl furoxans were successfully probed with this method which is more sensitive than the UV-Vis based Griess Reagent method. With the Griess Reagent method, it was found that 2-methyl derivative gave the highest absorbance compared to others.

Probing nitroxide release from dibenzoyl furoxans using a fluorescent assay

243rd National Meeting (2012)

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