Capillary high performance liquid chromatography is a separation technique used widely in biochemistry and analytical chemistry in applications such as characterization of unknown mixtures and proteome research, mostly in combination with mass spectrometry. A monolith is a single piece of material with interconnected skeletons and highly permeable throughpores, which serves as a very efficient separation medium in liquid chromatography. Monolithic columns typically consist of silica or cross-linked organic polymer. In this study, photopolymerized organo-silica hybrid monolith was prepared using simultaneous sol-gel transition and photopolymerization of a mixture of 3-(trimethoxysilyl)propyl methacrylate (MPTMS),  an aqueous acid catalyst (0.12 M HCl), a porogen (toluene), and a photoinitiator (2,2'-azobisbutyronitrile, AIBN). This homogeneous solution was irradiated with ultraviolet light at 365nm during the sol-gel transition directly within the confines of a pretreated fused silica capillary (100 �m I.D.). The resulting porous monolith enabled reversed-phase liquid chromatography of alkylbenzenes in reversed-phase mode.

Preparation of organo-silica monolithic column for application in capillary liquid chromatography utilizing 2,2′-azobisbutyronitrile at 365 nm

243rd National Meeting (2012)

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.