There is much epidemiological evidence that diets rich in fruit and vegetables tend to show protective effect against cardiovascular diseases, aging, cancer, diabetes. This protective effect is hypothesized to be due to polyphenols, which are the major antioxidants in plant foods.  Polyphenols are extensively metabolized and these metabolites may act as the active ingredients. Pure polyphenols like quercetin, catechin and hesperetin were injected through intraperitoneal injections into male rats at doses ranging from 50mg/kg of body weight daily for 3 days and were sacrificed 2 hours after the last injection. The liver, kidney, heart, brain and prostate were harvested. Homogenized solutions of these organs were prepared for further analysis with HPLC and LC-MS for monitoring the polyphenols. HPLC tentatively identified the presence of aglycone polyphenols and their metabolites in the extracts. The LC-MS method demonstrated necessary sensitivity and selectivity to confirm and quantify polyphenols and their metabolites in the extracts.

Polyphenol aglycones are the predominant compounds found in rat organs after supplementation with pure polyphenols via intraperitoneal injection

243rd National Meeting (2012)

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