Many solid tumor types, including breast and colon cancer, are found to contain small proportions of cells that are capable of proliferation, self-renewal, and differentiation into the various cell types seen in bulky tumors. These cells, termed �cancer stem cells� (CSCs), may be responsible for treatment resistance and tumor relapse. Several methods have been established to isolate CSCs from bulky tumors, including suspension culture and surface marker-based cell sorting. Metabolic labeling of proteins with non-canonical amino acids combing with fluorogenic probe provides powerful tools to study spatial and temporal variation in protein profile. In this work, the methionine analogue homopropargylglycine (HPG) was used to metabolic labeling of new synthesized proteins in isolated CSCs and non-CSCs. A fluorogenic probe reacted with HPG after labeling by �Click chemistry� to provide visual signals. Our study will help to identify difference between CSCs and non-CSCs in protein level in a spatial and temporal manner.

Characterization of protein profile in cancer stem cells by   noncanonical amino acids

243rd National Meeting (2012)

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.