Recently, a commercially available boron-doped diamond (BDD) working electrode has been developed to use with HPLC. Advantages of using BDD versus a carbon electrode are that BDD electrodes have a higher working potential, less electrode fouling, and decrease noise making them preferred for compounds with higher redox potentials.  The objective of this work is to evaluate the commercially available BDD working electrode by evaluating the test analytes adenosine and dopamine, which have biological significance. Hydrodynamic voltammograms were constructed for each analyte of interest and the optimal applied potential for dopamine and adenosine was +920 mV versus a palladium reference electrode, respectively. The range of concentration detection achieved with the BDD electrode for dopamine is 10 nM to 750 nM, and for adenosine is 3 nM to 50 nM.  Based on these preliminary findings, the study will be extended to develop a separation method of dopamine and adenosine for brain homogenates.

Simultaneous detection of dopamine and adenosine using a boron-doped diamond electrode

243rd National Meeting (2012)

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