In this contribution, we designed a novel siRNA delivery system combined the advantages of mesoporous silica nanoparticles (MSNs) and the RNA interference technology. The siRNA, to interference survivin gene overexpressed in numerous tumor types, was loaded into the channel of amino-modified MSNs where the siRNA can be free from enzymatic degradation, maximal adsorption capacity up to 125 �g/ml. The synthesized spherical particles with excellent monodisperse characteristic facilitated drug uptake with the optimal particles size of ca. 120 nm. The results of cellular experiments presented that the vectors with high concentration siRNA could be internalized by more than 95% Hela or HepG2 cells. MTT assay results indicated that there is little cytotoxicity by the presence of MSNs for loadings of up to 200 �g/ml. After MSNs were surface-functionalized with transferin and folic acid as cell penetrating agent, the efficiency of internalization and apoptosis of tumor cells was further improved.

Designed a novel siRNA delivery system using the monodisperse mesoporous silica nanoparticles as nanovehicle

243rd National Meeting (2012)

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