Supramolecular interactions play a pivotal role in assembling smaller molecular components into functional architectures, which can be engineered to function in biological systems. We describe here the use of synthetic supramolecular host-guest interaction for highly efficient bioorthogonal labeling of cellular targets. �-cyclodextrin modified antibodies against targets of interest were used as scaffolds to bind adamantane linked nanoparticles onto live cells via a host-guest interaction. Compared to conventional labeling techniques, the supramolecular interaction-based approach showed much higher efficiency and robustness in cellular labeling. Furthermore, we show that this technique has broad utility for a range of biodiagnostic applications, including magnetic sensing, immunostaining and cell sorting. This supramolecular strategy represents a new class of coupling platform that not only increases targeting capabilities but also extends detection limits by improving labeling efficiency.

Bioorthogonal nanoparticle labeling and detection of biomarkers through supramolecular host-guest interaction

243rd National Meeting (2012)

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