The use of magnetic materials is expected to afford significant advances in cell separation, diagnostics, and therapy of scant cell population. We describe a set of highly sensitive and multiplexable magnetic tagging method that amplifies the loading of magnetic nanoprobes (MNPs) onto targeted cells. The methods utilize oligonucleotides, copper-free click chemistry, and supramolecular interaction as the binding agents between the functionalized antibody and the modified MNP. We validated the method by surface plasmon resonance measurements, labeling of specific growth factor receptor, and detection of human cancer cells. The efficiency of these methods was demonstrated by profiling various cancer markers of interest (HER2/neu, EpCAM, and EGFR), each of which exhibit varying abundance levels, on a panel of cancer cell lines. Not only did this method allow multiplexed analysis, but it was also capable of detecting multiple markers in a single sample containing only a small number of cells.

Multiplexed labeling and signal amplification in diagnostic magnetic resonance

243rd National Meeting (2012)

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