This project aims to obtain Hanford Site microorganisms and characterize their phylogeny, biotransformation rates, electron donor utilization, and identify putative terminal reductase proteins involved in Fe redox reactions.  To isolate microorganisms able to reduce Fe(III)-oxides either directly or indirectly, sediment and groundwater samples were collected for microcosm construction.  Microcosms were amended with synthetic groundwater that closely matched site geochemistry and demonstrated that organic acids and alcohols supported the reduction of Fe(III)-oxides.  Fe(III)-oxide reduction was also influenced by biotic interactions with components of Hanford groundwater, including sulfate.  We have isolated two axenic Fe(III)-oxide reducing cultures of Azospira sp. selected from either acetate�amended groundwater or ethanol�amended sediment microcosms collected at the oxic/anoxic transition of the upper fine-grained Ringold Formation.  Characterization studies will enable us to better understand the biological processes influencing contaminant mobility at the Hanford Site.  This research is part of the SBR SFA at Pacific Northwest National Laboratory.

Isolation and characterization of metal redox-transforming microorganisms from the   Hanford Site 300 Area

243rd National Meeting (2012)

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