NO and TNF-a are associated with many inflammatory diseases. In this study, we investigated the effects of a beta-glucan from Lentinus edodes (LNT-S) on the NO and TNF-a production in LPS-stimulated murine RAW264.7 macrophages. The results suggested that treatment with LNT-S not only resulted in the striking inhibition of TNF-a and NO production in LPS-activated macrophage RAW264.7 cells, but also the protein expression of iNOS and the gene expression of iNOS mRNA and TNF-a mRNA. It is surprising that LNT-S enhanced LPS-induced NF-kB p65 nuclear translocation and NF-kB luciferase activity, but severely inhibited the phosphorylation of JNK1/2 and ERK1/2. The neutralizing antibodies of anti-Dectin-1 and anti-TLR2 hardly affected the inhibition of NO production. All these results suggested that the suppression of LPS-induced NO and TNF-a production was at least partially attributable to the inhibition of JNK1/2 and ERK1/2 activation.

Lentinan inhibits NO and TNF-a production and MAP kinases in RAW264.7 macrophages

243rd National Meeting (2012)

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