Challenges exist in the propagation of human pluripotent stem cells (hPSCs) due to their ability to spontaneously differentiate and accumulation of chromosomal abnormalities. Recent studies from our laboratory have shown that extracellular matrix (ECM) based substrates generated from human fibroblasts successfully maintained hPSCs in their undifferentiated state for extended periods. We also conducted proteomic analyses to identify the ECM proteins in mouse embryonic- and two human fibroblast-derived ECM-based substrates. These studies form the basis for identification of appropriate ECM components to generate defined substrates that synergistically promotes activation of adhesion and signaling pathways responsible for hPSC self-renewal.

Characterization of extracellular matrix components towards generation of defined substrates for human pluripotent stem cell propagation

243rd National Meeting (2012)

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