DNA seems to have both negative and positive purposes. Addressing the negative, virus dsDNA is one of many of their modes in which they are able to infect hosts. A methodology was developed for characterizing the dsDNA under variable temperature NMR spectroscopy in order to provide a basis for future experimentation. In order to accomplish this the scientific instrument NMR was used, allowing atoms and their interactions to be quantified. A twenty-one base pair dsDNA Oligo derived from the Homo Sapiens microRNA 155 containing a 2' flouro substituent was analyzed via NMR spectroscopy for the temperatures ranges from 25- 75�C to observe the dynamic DNA bonding. In order to better understand the DNA bond interactions, 1H NMR and 19F NMR scans were used. The NMR spectras revealed the uncoiling of DNA as it melted and the corresponding molecular interactions.

Dynamic DNA hydrogen bonding detection using variable temperature nuclear magnetic resonance spectroscopy

243rd National Meeting (2012)

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